NAD+ 500mg

What is NAD+ 500mg?

Nicotinamide adenine dinucleotide occupies a position of unparalleled importance in cellular biochemistry. This dinucleotide serves simultaneously as an electron carrier in redox reactions (the NAD+/NADH couple drives glycolysis, the TCA cycle, and oxidative phosphorylation), a substrate for sirtuins (deacetylases that regulate metabolism and longevity pathways), a substrate for PARPs (DNA repair enzymes), and a precursor for calcium signaling molecules. Without adequate NAD+, cellular energy production, gene expression regulation, and DNA maintenance all collapse.

NAD+ levels decline with age across tissues, correlating with metabolic dysfunction and cellular senescence—a relationship that has positioned NAD+ at the center of aging research. Studies investigating sirtuins, researching cellular energy metabolism, exploring DNA repair mechanisms, or modeling age-related NAD+ decline require direct NAD+ application or measurement.

Mechanism of Action

NAD+ functions as a critical electron carrier in cellular redox reactions, serving as the oxidizing agent in glycolysis, the citric acid cycle, and the electron transport chain. Beyond its role in energy metabolism, NAD+ serves as substrate for multiple regulatory enzyme families including sirtuins (protein deacetylases), poly(ADP-ribose) polymerases (PARPs involved in DNA repair), and CD38 (cyclic ADP-ribose synthase). These NAD+-consuming enzymes link cellular energy status to gene expression, DNA repair, and calcium signaling.

Age-related NAD+ decline compromises these regulatory pathways, contributing to mitochondrial dysfunction, genomic instability, and metabolic dysregulation. NAD+ supplementation restores activity of NAD+-dependent enzymes: sirtuins enhance mitochondrial biogenesis and stress resistance, PARPs maintain genomic integrity, and improved electron transport chain function enhances cellular energy production. This makes NAD+ a central hub connecting metabolism, epigenetics, and cellular maintenance—positioning it as a key research tool for studying aging and metabolic disease mechanisms.

Key Research Findings

• Restores mitochondrial function and enhances cellular energy metabolism through effects on electron transport chain efficiency and sirtuin-mediated mitochondrial biogenesis (Yoshino et al. Cell Metab 2018;27(3):513-28. PMID: 29249689)
• Supports DNA repair mechanisms through PARP activation and maintains genomic stability under oxidative stress conditions
• Declines significantly with age across multiple tissues, with restoration improving metabolic function and stress resistance in aging models

Research Applications

• Redox biochemistry
• Sirtuin activation research
• Cellular energy metabolism
• PARP enzyme function
• Mitochondrial studies
• NAD+/NADH ratio measurement
• Cellular aging mechanisms
• DNA repair pathways

Reconstitution & Use

Reconstitute with bacteriostatic water for laboratory use. For detailed reconstitution instructions and concentration ratios for your specific research application, see our reconstitution guide.

Storage & Handling

Store lyophilized at -20°C protected from light (NAD+ is photosensitive). Upon reconstitution with sterile water, use immediately or store at 2-8°C for up to 30 days. Avoid repeated freeze-thaw cycles which degrade the dinucleotide.